抗体GAPDH Polyclonal antibody

经过测试的应用

Positive WB detected inhuman placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells

Positive IP detected inA549 cells

Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected inHeLa cells, HepG2 cells

Positive FC detected inHEK-293 cells

Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

推荐稀释比

APPLICATIONDILUTION

Western Blot (WB)WB : 1:5000-1:40000

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunohistochemistry (IHC)IHC : 1:200-1:800

Immunofluorescence (IF)IF : 1:500-1:2000

Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

发表文章中的应用

WBSee 5425 publications below

IHCSee 7 publications below

IFSee 8 publications below

IPSee 13 publications below

ELISASee 2 publications below

CoIPSee 10 publications below

RIPSee 2 publications below

产品信息

10494-1-AP targets GAPDH in WB, RIP, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn , cabbage, rice samples.

Tested ApplicationsFC, IF, IHC, IP, WB, ELISA

Cited ApplicationsCell Treatment, CoIP, ELISA, IF, IHC, IP, RIP, WB

Tested ReactivityHuman, Mouse, Rat, Pig, Arabidopsis , Corn , Cabbage, Rice

Cited ReactivityHuman, Rat, Goat, Treeshrew, Chicken, Yeast, Leech, Arabidopsis, Zebra Finches, Sheep

ImmunogenGAPDH fusion protein Ag0766 种属同源性预测

Host / IsotypeRabbit / IgG

ClassPolyclonal

TypeAntibody

Full Nameglyceraldehyde-3-phosphate dehydrogenase

SynonymsG3PD, GAPD, GAPDH, OK/SW cl.12

Calculated molecular weight36 kDa

Observed molecular weight36 kDa

GenBank accession numberBC004109

Gene symbolGAPDH

UniprotP04406

Gene ID (NCBI)2597

RRIDAB_2263076

ConjugateUnconjugated

FormLiquid

Purification MethodAntigen affinity purification

Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.