抗体HA tag Polyclonal antibody

经过测试的应用

Positive WB detected inrecombinant protein, Transfected HEK-293 cells

Positive IP detected inTransfected HEK-293 cells

Positive IF detected inTransfected HEK-293 cells

推荐稀释比

APPLICATIONDILUTION

Western Blot (WB)WB : 1:5000-1:10000

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunofluorescence (IF)IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

发表文章中的应用

WBSee 442 publications below

IHCSee 3 publications below

IFSee 39 publications below

IPSee 131 publications below

FCSee 1 publications below

CoIPSee 33 publications below

ChIPSee 6 publications below

产品信息

51064-2-AP targets HA tag in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested ApplicationsIF, IP, WB, ELISA

Cited ApplicationsChIP, CoIP, FC, IF, IHC, IP, WB

Tested ReactivityRecombinant Protein

Cited ReactivityHuman, Mouse, Rat, Chicken, Monkey, Zebrafish, Duck, Canine

ImmunogenPeptide 种属同源性预测

Host / IsotypeRabbit / IgG

ClassPolyclonal

TypeAntibody

Full NameHA tag

SynonymsHA tag, YPYDVPDYA

Calculated molecular weight1 kDa

Gene symbol

Gene ID (NCBI)

RRIDAB_11042321

ConjugateUnconjugated

FormLiquid

Purification MethodAntigen affinity purification

Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.