抗体DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

经过测试的应用

Positive WB detected inTransfected HEK-293 cells

Positive IP detected inTransfected HEK-293 cells

Positive IF detected inTransfected HEK-293 cells

推荐稀释比

APPLICATIONDILUTION

Western Blot (WB)WB : 1:5000-1:50000

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunofluorescence (IF)IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

发表文章中的应用

WBSee 99 publications below

IHCSee 1 publications below

IFSee 15 publications below

IPSee 32 publications below

CoIPSee 23 publications below

ChIPSee 3 publications below

RIPSee 1 publications below

产品信息

66008-4-Ig targets DYKDDDDK tag in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested ApplicationsIF, IP, WB, ELISA

Cited ApplicationsChIP, CoIP, IF, IHC, IP, RIP, WB

Tested ReactivityRecombinant Protein

Cited ReactivityHuman, Mouse, Chicken, Zebrafish, Pig, Canine, Bovine

ImmunogenPeptide 种属同源性预测

Host / IsotypeMouse / IgG2b

ClassMonoclonal

TypeAntibody

Full NameDYKDDDDK tag

SynonymsDDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag

Gene symbol

Gene ID (NCBI)

RRIDAB_2918475

ConjugateUnconjugated

FormLiquid

Purification MethodProtein A purification

Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.